关键词: 高血压并发抑郁, 慢性不可预知温和应激, 孤养, 自发性高血压大鼠, 模型

Abstract: Objective To observe the effects of chronic unpredictable mild stress ( CUMS) combined with solitary rearing on the behavior, serum 5-HT and neurons in the hippocampus of spontaneously hypertensive rats ( SHR) . Method Twenty SHR rats were randomly divided into SHR group and model group, and the same number of Sprague-Dawley ( SD) rats were set as the control group. Predictive stress (CUMS) combined with solitary rearing was used to intervene for 5 weeks, and the rest of the groups were fed normally. The caudal arterial blood pressure of rats in each group was measured on the 14th day and at the end of modeling, respectively. Open-field experiment and sugar water consumption experiment were performed. Forced swimming experiment was conducted after modeling, 5-HT content in serum was detected by ELISA, and activity and arrangement of hippocampal neurons and the number of neurons’ Nissl bodies were observed by HE staining and Nissl staining. Result Compared with the control group, the blood pressure of SHR group increased significantly ( P < 0. 01) , body weight growth rate increased obviously(P<0. 01) , the level of activity and the number of upright, sugar water consumption rate and forced swimming fixed time, 5-HT content there was no significant difference (P>0. 05) , roundness, hippocampal neurons form order, nissl bodies and more dense; Systolic blood pressure of model group was significantly increased (P< 0. 01) , body weight growth rate was not significantly different ( P > 0. 05) , horizontal activity and upright times, sugar water consumption rate were significantly decreased (P<0. 05, P<0. 01) , forced swimming immobile time was significantly increased (P<0. 01) , 5-HT content was significantly decreased (P<0. 01) . The arrangement of hippocampal neurons was loose and the nite body was apoptotic. Compared with SHR group, systolic blood pressure in model group was not significantly increased (P>0. 05) , body weight growth rate, horizontal activity and upright times, sugar water consumption rate were significantly decreased ( P<0. 01) , the immobile time of forced swimming was significantly increased ( P < 0. 01 ) , 5-HT content was significantly decreased ( P < 0. 01 ) , the arrangement of hippocampal neurons was loose, and Nissl bodies apoptosis. Conclusion Chronic unpredictable stress (CUMS) combined with solitary rearing induced depression-like changes in behavior, neurotransmitters and hippocampal neurons in SHR rats.

Key words: hypertension complicated with depression, chronic unpredictable mild stress, solitary rearing, SHR, model